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Molecouler
Labgenomics
The LabGunTM COVID-19 ExoFast RT-PCR Kit contains a specific ready-to-use system for the detection of the SARS-CoV-2 using RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the real-time PCR system. The targets (RdRp and N genes) and Internal Control are amplified in a manner of multiplex real-time RT-PCR in one tube. The first step is a reverse transcription (RT), during which the SARS-CoV-2 RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by PCR. Fluorescence is emitted and measured in real-time by the optical unit of a real-time PCR instrument during the PCR step. In addition, the kit contains a system to identify RNA extraction effectiveness and possible PCR inhibition by amplifying the internal control in the same reaction.
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